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KMID : 1161520160200060363
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Animal Cells and Systems
2016 Volume.20 No. 6 p.363 ~ p.368
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Neuroprotective effect of Korea Red Ginseng extract on 1-methyl-4-phenylpyridinium-induced apoptosis in PC12 Cells
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Ryu Sun
Koo Sung-Tae
Ha Ki-Tae
Kim Seung-Tae
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Abstract
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Background: Korean Red Ginseng (KRG) is a valuable herb in Asian countries that is used as a crude substance to inhibit inflammation and to enhance vitality, longevity and immunity. The protective effects of KRG against the toxicity of 1-methyl-4-phenylpyridinium (MPP+) were investigated in vitro in the present study.
Methods: PC12 cells were pretreated with the water extract of KRG for 24?h, then incubated with MPP+ for 24?h. The growth of the cells was assessed using a live cell viability assay, the ratio of apoptotic cells was measured using flow cytometry and morphology of the apoptotic cells was detected using terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) staining. The expressions of caspase-3 and -9 were measured by quantitative real-time PCR.
Results: Pretreatment of the KRG extract increased cell viability significantly when compared with that of only MPP+-treated cells (p?
Conclusion: These results indicate that KRG treatment suppresses MPP+-induced apoptosis in PC12 cells by regulating caspase cascades, suggesting a possible role for KRG in the prevention and treatment of Parkinson’s disease.
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KEYWORD
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Parkinson’s disease, 1-methyl-4-phenylpyridine (MPP+), apoptosis, Panax ginseng, PC12 cells
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